おすすめの製品
包装
pack of 500 mL
メーカー/製品名
Cytiva 17-3712-03
Matrix
highly cross-linked 6% agarose
平均直径
90 μm
cleaning in place
2-14
動作範囲
2-13
キャパシティ
≥10 mg binding capacity (histidine-tagged protein)
関連するカテゴリー
詳細
EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.
His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.
Convenient prepacked columns:
- HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
- His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.
特徴および利点
- Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
- Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
- Retained binding capacity of up to 10 mg/mL resin.
- Simplified sample handling – fewer steps needed compared with conventional workflow.
- Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
最新バージョンのいずれかを選択してください:
資料
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
プロトコル
This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)