F4772

モノクロナール抗サイトケラチンペプチド18

clone CY-90, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-Cytokeratin Peptide 18
• MDL番号: MFCD00162250
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: CY-90, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: wide range
• テクニック: direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KRT18(3875) , KRT18(3875)

詳細

詳細

Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues.

FITC Monoclonal Anti-Cytokeratin Peptide 18 is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the CY-90 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratin 18 (CYK18) is a 45 kDa polypeptide, differentially expressed in various human tissues.

免疫原

ヒト表皮癌由来A-431細胞およびヒト乳ガン細胞株MCF-7。

アプリケーション

Monoclonal Anti-Cytokeratin Peptide 18 FITC antibody produced in mouse has been used in:

• direct immunofluorescent staining
• immunohistochemistry
• double immunofluorescence
• immunocytochemical and immuno?electron microscopic localization of keratins

Monoclonal Anti-Cytokeratin Peptide 18-FITC antibody:

• is suitable for immunohistochemistry to identify renal tubular epithelial cells in the extrarenal cells regeneration during acute renal failure
• is suitable for immunocytochemical and immuno-electron microscopic localisation of keratins in human materno-foetal interaction zone
• may be used for single or double labeling procedures

生物化学的／生理学的作用

Cytokeratins also called keratins (Ks) according to the new nomenclature system.These are known to be the major structural proteins of epithelial cells. It is heteropolymeric in nature i.e. in cells more than one keratin protein is always present. It belongs to type I acrylic sub-family and is differentially expressed in various human tissues. It maintains the structural integrity of extra-embryonic membrane epithelia. Broadly keratins are grouped into two types on the basis of their biochemical and immunological properties: Type II keratins are a family of basic proteins identified as K1 to K8, whereas type I keratins consists of K9 to K20, belong to the acidic group.

Monoclonal anti-cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. FITC Monoclonal Anti-Cytokeratin Peptide 18 is a chain specific antibody which can facilitate typing of normal, metaplastic and neoplastic cells. Cytokeratin 18 (CYK18) is a cytoskeletal protein associated with apoptosis. CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.

物理的形状

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA with 15 mM sodium azide as a preservative.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable