F4771

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse

clone 1B10, ascites fluid

• 別名: Fibroblast Surface Protein Antibody, Fibroblast Surface Protein Antibody - Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse
• MDL番号: MFCD00164646
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 1B10, monoclonal
• 分子量: antigen 43-72-80 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: human
• テクニック: complement-mediated cytotoxicity assay: 1:500; flow cytometry: suitable; immunohistochemistry (frozen sections): suitable
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Monoclonal Anti-Human Fibroblast Surface Protein (mouse IgM isotype) is derived from the 1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cultured human thymic fibroblasts. Human fibroblast surface protein is a fibroblast antigen that is expressed mainly on human synovial, foreskin and thymic fibroblasts and is absent on human epithelial cells and lymphocytes.

免疫原

培養ヒト胸腺繊維芽細胞

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-Fibroblast Surface Protein antibody produced in mouse has been used in:

• immunofluorescence
• flow cytometry
• immunohistochemistry
• immunoblotting

Monoclonal anti-fibroblast surface protein antibody can be used in flow cytometry of human fibroblasts and monocytes. It is also useful in immunoblotting and western blotting.

生物化学的／生理学的作用

Monoclonal Anti-Fibroblast Surface Protein antibody is also used to remove fibroblasts from human cultured cells by inhibiting fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement. In addition, apart from the functional significance of cells characteristics, the characterization of cell lines is important for correlation of cultures with tissue origin, identification of the lineage to which the cells belong or the precursor status of the cells. Monoclonal antibodies to fibroblast surface protein should provide a powerful tool for control of fibroblast growth in a variety of human cell culture systems, such as thymic epithelial (TE) cell cultures allowing the growth of highly enriched populations of the TE cells.

ターゲットの説明

この繊維芽細胞抗原は、ヒト滑膜、乳腺、包皮、胸腺の繊維芽細胞や悪性線維肉腫の組織で明らかにされています。抗原は、細胞膜とリソソ-ムの両方に結合しています。

アナリシスノート

補体を介した微量細胞傷害性試験での使用希釈率は1:500以上です。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable