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We provide innovative solutions for academic research, biotech research and pharmaceutical drug discovery. Our excellent systems, equipment, and personnel provide the highest quality tools that you require.
Custom Cloning - Now with TurboGFP™
There may be times when classic drug selection for stable or semi-stable cell lines is not possible or desired. In addition, orthogonal methods of selection from puromycin may be required for the expression of multiple shRNAs or expression of shRNA concomitant with transgene expression.
We now offer vectors containing tGFP as a solution to these problems. This vector maintains all of the other elements of the pLKO.1 vector, but the puromycin resistance gene has been replaced by TurboGFP. Our MISSION bio-production team will customize the vector with either a TRC shRNA sequence or your favorite shRNA sequence. With TurboGFP in the vector, FACS and fluorescence microscopy techniques allow for rapid estimation of transduction efficiency and automated, rapid selection of cell populations that stably express the shRNA. Selection by GFP provides an orthogonal method when cells already stably express puromycin resistance. In addition, we also offer pLKO.1-puro-CMV-tGFP. With this vector, you can choose between GFP and puromycin selection, all with the shRNA expression vector.
For selection by a second class of antibiotics, we offer vectors containing the neomycin resistance gene. These two vectors are pLKO.1-hPGK-Neo and pLKO.1-CMV-Neo. Stable shRNA integrants can be found by selecting in G418 (Product Number A1720). As with puromycin, a cytotoxicity profile for your particular cell line will need to be generated. As an example, see the puromycin protocol, but utilize G418 instead of puromycin. As a starting point, 1 mg/mL of G418 has been utilized for selection of shRNA constructs in A549 cells.
The service includes cloning, sequencing, DNA preparation (500 ng) and lentiviral preparation (200 μl at 1 x 106 VP/mL, minimum titer via p24 assay). The DNA and viral preparations are available to be shipped 4-6 weeks from the time the order is placed. For ordering, visit our custom shRNA ordering page or contact our sales representative.
Large-Scale and High Titer Lentiviral Particles
We have a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 μl of 1 x 106 VP/mL (via p24 assay) minimum titer. Any clone, target set, or control can be produced at larger volumes with the same minimum titer specification or higher titers. Our highest titer option is 109 VP/mL. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Our robust and optimized processes will save you time and reduce variability in your transduction experiments. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form.
Custom Cloning - Now with TurboGFP™
There may be times when classic drug selection for stable or semi-stable cell lines is not possible or desired. In addition, orthogonal methods of selection from puromycin may be required for the expression of multiple shRNAs or expression of shRNA concomitant with transgene expression.
We now offer vectors containing tGFP as a solution to these problems. This vector maintains all of the other elements of the pLKO.1 vector, but the puromycin resistance gene has been replaced by TurboGFP. Our MISSION bio-production team will customize the vector with either a TRC shRNA sequence or your favorite shRNA sequence. With TurboGFP in the vector, FACS and fluorescence microscopy techniques allow for rapid estimation of transduction efficiency and automated, rapid selection of cell populations that stably express the shRNA. Selection by GFP provides an orthogonal method when cells already stably express puromycin resistance. In addition, we also offer pLKO.1-puro-CMV-tGFP. With this vector, you can choose between GFP and puromycin selection, all with the shRNA expression vector.
For selection by a second class of antibiotics, we offer vectors containing the neomycin resistance gene. These two vectors are pLKO.1-hPGK-Neo and pLKO.1-CMV-Neo. Stable shRNA integrants can be found by selecting in G418 (Product Number A1720). As with puromycin, a cytotoxicity profile for your particular cell line will need to be generated. As an example, see the puromycin protocol, but utilize G418 instead of puromycin. As a starting point, 1 mg/mL of G418 has been utilized for selection of shRNA constructs in A549 cells.
The service includes cloning, sequencing, DNA preparation (500 ng) and lentiviral preparation (200 μl at 1 x 106 VP/mL, minimum titer via p24 assay). The DNA and viral preparations are available to be shipped 4-6 weeks from the time the order is placed. For ordering, visit our custom shRNA ordering page or contact our sales representative.
Large-Scale and High Titer Lentiviral Particles
We have a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 μl of 1 x 106 VP/mL (via p24 assay) minimum titer. Any clone, target set, or control can be produced at larger volumes with the same minimum titer specification or higher titers. Our highest titer option is 109 VP/mL. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Our robust and optimized processes will save you time and reduce variability in your transduction experiments. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form.
弊社の網羅的なshRNA製品は、15,000種類のヒト遺伝子と15,000種類のマウス遺伝子をターゲットとした150,000種類を超えるshRNAプレクローンコンストラクトを基盤としています。shRNAライブラリーは、遺伝子ファミリーセット、キナーゼやイオンチャネルなどの特定の機能に関連する遺伝子コレクション、遺伝子ターゲットセット、任意の遺伝子をターゲットとするshRNAクローンセット、個別のshRNAクローンとして販売しています。弊社のshRNAとレンチウイルスの生産プラットフォームには、お客様のRNAi研究のニーズに対応できる優れた品質とサービスを提供するため、最新のロボット技術、液体処理技術、研究室情報中央管理システム(LIMS)を採用した専用研究室を備えています。
その他情報
弊社のカスタムshRNAサービスをご注文いただく際は、カスタムshRNAサービスをご利用いただくか、MISSIONRNAi@sial.comまでeメールにてご連絡ください。
プロトコールとアプリケーションデータに関しては、sigma.com/shrnaをご覧ください。
法的情報
この製品の使用は、ひとつ以上のライセンス契約の対象となります。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
カルタヘナ法
カルタヘナ法
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
MISSION® Lentiviral shRNA
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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