C6223

Monoclonal Anti-CXCR-1 (IL-8 RA) antibody produced in mouse

clone 42705.111, purified immunoglobulin, lyophilized powder

• MDL番号: MFCD02096157
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 42705.111, monoclonal
• 形状: lyophilized powder
• 化学種の反応性: human
• テクニック: flow cytometry: 10 μg/mL using using human blood cells (10 μL diluted antibody/10⁵-10⁶ cells); immunohistochemistry (frozen sections): 25 μg/mL using human tissue (speen); neutralization: suitable
• アイソタイプ: IgG2a
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IL8RA(3577) ; mouse ... Il8ra(227288)

詳細

詳細

Chemokine receptor CXCR1 also refers as type I IL-8 receptor or IL-8RA is a class A, rhodopsin-like G-protein-coupled receptor, expressed mainly in neutrophils but not in T or B lymphocytes . It has a crucial role in cellular signal transduction and targeted as drug receptors. Monoclonal anti-CXCR-1 (IL-8 RA) antibody can be used to neutralize the bioactivity facilitated by human cell surface CXCR-1. Mouse anti-CXCR-1 (IL-8 RA) antibody reacts specifically with CXCR-1 transfected cells but not with the parent cell line. The product has also shown no cross-reactivity with human CXCR-2.

特異性

The antiboody, in the presence of anti-CXCR2 (Sigma Product No. C6348), will neutralize human cell surface CXCR-1 mediated-myloperoxidase release from human granulocytes induced by IL-8. The antibody reacts with human CXCR1 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CXCR2.

免疫原

human CXCR1 transfected NSO mouse myeloma cells.

アプリケーション

Monoclonal anti-CXCR-1 (IL-8 RA) antibody can be used in flow cytometry for detecting CXCR-1 present on human blood cells. It can also be used in immunohistochemistry (25 μg/mL) using frozen human tissue sections (spleen).

物理的形状

凍結乾燥品(PBS溶液, 5%トレハロース含有, 0.2 μmフィルター滅菌済み)。

調製ノート

Purified using protein G.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable