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Merck

N6655

Sigma-Aldrich

抗神経成長因子2.5S ウサギ宿主抗体

fractionated antiserum, buffered aqueous solution

別名:

抗NGF-2.5S抗体

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About This Item

EC Number:
MDL番号:
UNSPSCコード:
51111800
NACRES:
NA.41

由来生物

rabbit

品質水準

結合体

unconjugated

抗体製品の状態

fractionated antiserum

抗体製品タイプ

primary antibodies

クローン

polyclonal

フォーム

buffered aqueous solution

化学種の反応性

mouse, human

テクニック

Ouchterlony double diffusion: suitable
western blot: 1:2,000

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... NGF(4803)
mouse ... Ngf(18049)

詳細

Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.

特異性

NGF (2.5S、βおよび7S) のモニタリングに使用できます。ヒヨコ後根神経節やPC12細胞に対するNGFの生物学的作用を中和します。

免疫原

雄マウス顎下腺由来NGF-2.5S。

アプリケーション

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

生物化学的/生理学的作用

Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).

物理的形状

PBS溶液です。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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7S nerve growth factor has different biological activity from 2.5 S nerve growth factor in vitro
Shao N, et al.
Brain Research, 609(1-2), 338-340 (1993)
N Shao et al.
Brain research, 609(1-2), 338-340 (1993-04-23)
It is considered that two molecular forms of nerve growth factor (NGF), that is 7S NGF and 2.5S NGF have the identical neurotrophic effect on neurons. We now report that 7S NGF has different biological activities from 2.5S NGF in
Liang-Wei Chen et al.
The Journal of comparative neurology, 497(6), 898-909 (2006-06-28)
To address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/Bl mice that had been treated
Joshua S Barker et al.
Journal of toxicology and environmental health. Part A, 78(6), 397-407 (2015-03-04)
Previous studies demonstrated that interleukin-1β (IL-1β) and nerve growth factor (NGF) increase synthesis of substance P (SP) in airway neurons both after ozone (O3) exposure and by direct application. It was postulated that NGF mediates O3-induced IL-1β effects on SP.
New protein fold revealed by a 2.3-
McDonald N Q, et al.
Nature, 354(6352), 411-411 (1991)

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