C3556

カタラーゼ ヒト赤血球由来

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

• 別名: H₂O₂:H₂O₂ オキシドレダクターゼ
• CAS番号: 9001-05-2
• Enzyme Commission number: 1.11.1.6 (BRENDA, IUBMB)
• EC Number: 232-577-1
• MDL番号: MFCD00081483
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• 由来生物: human erythrocytes
• 品質水準: 200
• アッセイ: ≥90% (SDS-PAGE)
• 形状: buffered aqueous solution
• 比活性: ≥30,000 units/mg protein
• 分子量: tetramer ~250 kDa
• 濃度: ≤10 mg/mL
• テクニック: cell based assay: suitable
• UniProtアクセッション番号: P04040
• 輸送温度: wet ice
• 保管温度: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI Key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• 遺伝子情報: human ... CAT(847)

詳細

詳細

Research area: Cell Signaling

Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.

アプリケーション

Catalase from human erythrocytes has been used:

• to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.
• as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts
• as a component of the gloxy mix for single-molecule imaging

生物化学的／生理学的作用

Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.

カタラ-ゼは、活性酸素種である過酸化水素が水と酸素に分解する反応を活性化します。天然の抗酸化物質としても機能し、タンパク質、脂質および核酸の酸化的損傷から細胞を保護します。また、活性酸素種が遺伝子発現とアポト-シスに果たす役割に関する研究にも用いられています。

単位の定義

1ユニットは、pH 7.0、25°Cで、H₂O₂濃度が10.3 mMから9.2 mMに低下する間に1分あたり1.0 μmolのH₂O₂を分解する酵素量であり、A₂₄₀の減少率で測定します。

物理的形状

50 mM Trisバッファ-溶液、pH 8.0

保管および安定性

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

アナリシスノート

タンパク量はビウレット法で測定

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)