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Merck

32211

Sigma-Aldrich

クロロホルム

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

別名:

トリクロロメタン, 三塩化メチリジン

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About This Item

実験式(ヒル表記法):
CHCl3
CAS番号:
分子量:
119.38
Beilstein:
1731042
EC Number:
MDL番号:
UNSPSCコード:
12191502
PubChem Substance ID:
NACRES:
NA.04

認証

USP/NF
reag. ISO
reag. Ph. Eur.

品質水準

蒸気密度

4.1 (vs air)

蒸気圧

160 mmHg ( 20 °C)

グレード

puriss. p.a.

アッセイ

99.0-99.4% (GC)

形状

liquid

含みます

~1% ethanol as stabilizer

テクニック

RNA extraction: suitable

不純物

≤0.00001% free chlorine (Cl)
≤0.00005% free acid (as HCl)
≤0.0005% non-volatile matter
≤0.005% aldehydes and ketones (as CH3COCH3)
≤0.005% carbonyl compounds (as CO)
≤0.01% tetrachloroethene (GC)
≤0.01% tetrachloromethane (GC)
≤0.01% trichloroethene (GC)
≤0.01% water (Karl Fischer)
≤0.03% dichloromethane (GC)
0.6-1.0% ethanol (GC)

屈折率

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

密度

1.476-1.483 g/mL at 20 °C
1.492 g/mL at 25 °C (lit.)

微量陰イオン

chloride (Cl-): ≤1 mg/kg

微量陽イオン

Al: ≤0.5 mg/kg
B: ≤0.02 mg/kg
Ba: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.05 mg/kg
Sn: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES記法

ClC(Cl)Cl

適合性

complies for appearance
complies for reaction against H2SO4
complies for suitability of determ. w. dithizone

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI Key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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詳細

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO2 aqueous suspensions (wavelength range of 310-380nm).

アプリケーション

Chloroform may be employed as a solvent for the following studies:
  • Extraction of RNA from plant cells.
  • Preparation of dioleoylphosphatidylcholine (DOPC) solution.
  • Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).
It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.
Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.

その他情報

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table
The article number 32211-4X2.5L will be discontinued. Please order the single bottle 32211-2.5L which is physically identical with the same exact specifications.
The article number 32211-4X2.5L-M will be discontinued. Please order the single bottle 32211-2.5L-M which is physically identical with the same exact specifications.
The article number 32211-6X1L will be discontinued. Please order the single bottle 32211-1L which is physically identical with the same exact specifications.
The article number 32211-6X1L-M will be discontinued. Please order the single bottle 32211-1L-M which is physically identical with the same exact specifications.

ピクトグラム

Skull and crossbonesHealth hazard

シグナルワード

Danger

危険有害性の分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

ターゲットの組織

Central nervous system, Liver,Kidney

保管分類コード

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

引火点(°F)

does not flash

引火点(℃)

does not flash


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

PRTR

第一種指定化学物質

労働安全衛生法名称等を表示すべき危険物及び有害物

名称等を表示すべき危険物及び有害物

労働安全衛生法名称等を通知すべき危険物及び有害物

名称等を通知すべき危険物及び有害物

Jan Code

32211S-4X2.5L:4548173257037
32211-195L-D:
32211-6X1L:4548173256962
32211S-1L:4548173257013
32211JP-6X1L:4548173257006
32211-2.5L-C:
32211S-2.5L:4548173257020
32211-144X1L-D:
32211E-1L:
32211-195L:4548173256870
32211S-6X1L:4548173257044
32211-1L:4548173256887
32211-2.5L:4548173256894
32211-7L:4548173256986
32211-45L-D:
32211JP-4X2.5L:4548173256993
32211-25KG:4548173256917
32211-4X2.5L:4548173256955
32211-25KG-D:
32211-25L-C:
32211-18L:4548173256863
32211-40KG-D:
32211-72X2.5L:4548173256979
32211-250KG:4548173256900
32211-40KG:4548173256931
32211-BULK:
32211E-6X1L:
32211-250KG-D:
32211-45L:4548173256948
32211-25L:4548173256924
32211-144X1L:
32211-VAR:
32211-25L-D:


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Slide 1 of 8

1 of 8

Chloroform is not solvent but activator for cobalt complex catalyst of enantioselective borohydride reduction.
Kokura, Ai, et al.
Chemistry Letters (Jpn), 36(1), 26-27 (2006)
Gurman Singh Pall et al.
Nucleic acids research, 35(8), e60-e60 (2007-04-05)
The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and
G F Ames et al.
Journal of bacteriology, 160(3), 1181-1183 (1984-12-01)
We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the
Jameel A Feshitan et al.
Journal of colloid and interface science, 329(2), 316-324 (2008-10-28)
Microbubbles used as contrast agents for ultrasound imaging, vectors for targeted drug delivery and vehicles for metabolic gas transport require better size control for improved performance. Mechanical agitation is the only method currently available to produce microbubbles in sufficient yields
Z V Leonenko et al.
Biochimica et biophysica acta, 1509(1-2), 131-147 (2000-12-19)
We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES)

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