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由来生物
rabbit
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
分子量
calculated mol wt 135.34 kDa
observed mol wt ~135 kDa
精製方法
affinity chromatography
交差性
rat, mouse
包装
antibody small pack of 100 μg
テクニック
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG
エピトープ配列
C-terminal extracellular half
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
-10 to -25°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
rat ... Trpm4(171143)
詳細
Transient receptor potential cation channel subfamily M member 4 (UniProt: Q9ESQ5; also known as Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, LTrpC-4, LTrpC4, MLS2s, Melastatin-like 2) is encoded by the Trpm4 (also known as Ltrpc4) gene (Gene ID: 171143) in rat. TRPM4 is a multi-pass membrane protein with four cytoplasmic domains; six transmembrane domains; and four extracellular domains. Two isoforms of TRPM4 have been described that are produced by alternative splicing. Isoform 1 is highly expressed in the testis tissue with a moderate expression in brain, spleen, and thymus. Isoform 2 is expressed in the brain and spleen. It serves as a calcium-activated non-selective cation channel that mediates membrane depolarization. Although it is a calcium activated channel, it is impermeable to calcium. It mediates the transport of monovalent cations that leads to membrane depolarization. It is reported to participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL-2 production. TRPM4 is also involved in myogenic constriction of cerebral arteries. It plays a role in control of insulin secretion in pancreatic β-cells. It may also be involved in pacemaking activity or could cause irregular electrical activity under conditions of Calcium overload. It is known to affect Th1 and Th2 cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. This polyclonal antibody is reported toblock the channel vua binding to a region close to the channel porefrom extracellular space. Bu blocking TRPM4, it can downregulate TRPM4 surface expression and prevent hypoxia-induced cell swelling. (Ref.: Wei, S., et al. (2020). Front Cell Dev Bio. 8; 562584; Chen, B;et al. (2019). Pflugers Arch. 471(11-12); 1455-1466; Guinamard, R., et al. (2006). Hypertension. 48(4); 587-594; Launay, P., et al. (2002). Cell. 109(3); 397-407).
特異性
This rabbit polyclonal antibody detects Transient receptor potential cation channel subfamily M member 4 (TRPM4). It targets an epitope within 24 amino acids from the C-terminal, extracellular domain.
免疫原
GST-conjugated linear peptide corresponding to 28 amino acids between transmembrane segment 5 and 6 from the C-terminal half of rat TRPM4.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293 cells transfected with Mouse TRPM4.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRPM4 in lysate from HEK293 cells transfected with Mouse TRPM4.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected TRPM4 in Immunocytochemistry application (Low, S.W., et. al. (2021). Sci Rep. 11(1):10411).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected TRPM4 in rat spinal cord tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in lysate from HEK293 cells transfected with Mouse TRPM4.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRPM4 in lysate from HEK293 cells transfected with Mouse TRPM4.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected TRPM4 in Immunocytochemistry application (Low, S.W., et. al. (2021). Sci Rep. 11(1):10411).
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected TRPM4 in rat spinal cord tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
物理的形状
Purified rabbit polyclonal antibody in PBS without azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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