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Key Documents

安全性情報

AB1792P

Sigma-Aldrich

Anti-Dopamine D2 Receptor Antibody, cytoplasmic domain, long form

Chemicon®, from rabbit

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

affinity purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

化学種の反応性

canine, rat

メーカー/製品名

Chemicon®

テクニック

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

dry ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

特異性

Specific for Dopamine Receptor D2 long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (Dal Toso et al. 1989; Grandy et al. 1989; Monsma et al. 1990; Giros et al. 1989). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Boundy et al. 1993) The 27 aa rat D2RL peptide used for immunization was found only in the D2 long form and there is no significant homology with other dopamine receptors (D1, D2 short, and D3-D5).

SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 96% with human/bovine (26/27 aa), 62% with Xenopus (17/27 aa) D2L receptors. Antibodies generated to the rat sequence have been shown to recognize the canine D2R (Boundy et al. 1993). Antibody cross-reactivity with various species is not known.

免疫原

A 27 amino acid peptide sequence from the rat D2 long form within the cytoplasmic loop #3.
Epitope: cytoplasmic domain, long form

アプリケーション

Research Category
ニューロサイエンス
Research Sub Category
神経伝達物質及び受容体
Detect Dopamine D2 Receptor using this Anti-Dopamine D2 Receptor Antibody, cytoplasmic domain, long form validated for use in ELISA, IH, IP & WB.
Western blot: 1-10 μg/mL (Chemiluminescence technique) The predicted size of D2L form is approximately 50 kDa. However, additional bands at approximately 60 and 100 kDa, and others are typically seen in various tissues (Boundy, et al. 1993; Sakata, et al. 1992; Farooqui et al. 1991, 1992; Brock et al. 1992; Farooqui & Prasad 1992). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize various bands.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

Immunoprecipitation: 1-10 μg using 100-400 μg of protein (Boundy, et al. 1993).

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (AG211)/well)

Optimal working dilutions must be determined by the end user.

物理的形状

Affinity purified immunoglobulin. Liquid at 1.0 mg/mL in PBS, pH 7.4 with 0.1% BSA and 0.02% sodium merthiolate.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

AB1792P:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Abu Hasanat Md Zulfiker et al.
Journal of cellular biochemistry, 118(5), 979-993 (2016-06-14)
Recent evidences show that activation of serotonin 2A receptors (5-HT2A R) by agonists is significant in improving therapeutic activity of disease conditions, such as obsessive-compulsive disorder (OCD). Though the exact molecular mechanism is still not well understood, it is thought
Single and binge methamphetamine administrations have different effects on the levels of dopamine D2 autoreceptor and dopamine transporter in rat striatum.
Chauhan, H; Killinger, BA; Miller, CV; Moszczynska, A
International Journal of Molecular Sciences null
An antibody to dopamine D2 receptor inhibits dopamine antagonist and agonist binding to dopamine D2 receptor cDNA transfected mouse fibroblast cells.
Farooqui, S M and Prasad, C
Life Sciences, 51, 1509-1516 (1992)
B Giros et al.
Nature, 342(6252), 923-926 (1989-12-21)
Dopamine receptors are classified into D1 and D2 subtypes on the basis of their pharmacological properties and the intracellular responses they mediate. The cerebral D2 dopamine receptor is the target of drugs used to alleviate the main symptoms of schizophrenia.
Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum.
Farooqui, S M, et al.
Journal of Neurochemistry, 57, 1363-1369 (1991)

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