345386

Glucose Oxidase, Aspergillus niger, Recombinant

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

• 別名: Glucose Oxidase, Aspergillus niger, Recombinant
• CAS番号: 9001-37-0
• Enzyme Commission number: 1.1.3.4 (BRENDA, IUBMB)
• MDL番号: MFCD00131182
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• 品質水準: 100
• 詳細: Merck USA index - 14, 4460
• フォーム: lyophilized solid
• 比活性: ≥250 units/mg solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: yellow-brown
• 溶解性: 0.1 M phosphate buffer, pH 7.0: 5 mg/mL
• その他の活性: Amylase ≤0.1%; catalase ≤20 U/mg (dry weight); sucrase ≤0.1%
• 輸送温度: ambient
• 保管温度: 2-8°C
• SMILES記法: O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O
• InChI: 1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
• InChI Key: WQZGKKKJIJFFOK-VFUOTHLCSA-N

詳細

詳細

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

再構成

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

その他情報

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable