218799

Caspase-6, Human, Recombinant, E. coli

Caspase-6, Human, Recombinant is expressed as a single polypeptide in E. coli. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• 別名: Mch2
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• アッセイ: ≥95% (SDS-PAGE)
• 形状: lyophilized
• 比活性: ≥6000 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

M.W. 11,000 and 18,000 (heterodimer).

Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.

再構成

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to 1 unit/µl in PBS, 15% glycerol.

その他情報

LeBlanc, A., et al. 1999. J. Biol. Chem.274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable