189730

Streptavidin

• 別名: Streptavidin, Avidin, Streptomyces avidinii
• CAS番号: 9013-20-1
• MDL番号: MFCD00082035
• UNSPSCコード: 41116133

特性

• 形状: lyophilized solid
• 品質水準: 100
• 比活性: ≥10 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A high affinity biotin binding protein (K_d approximately 10^-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.

A high-affinity biotin binding protein.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.

Reconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.

その他情報

Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
Roth, J., et al. 1992. Histochemistry 98, 229.
Sano, T., et al. 1992. Science 258, 120.
Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
Srivastava, P., et al. 1992. Cytometry 13, 711.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable