94149

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

• 別名: Streptavidin-Atto 647N
• UNSPSCコード: 12161900
• NACRES: NA.25

特性

• 製品種目: BioReagent
• 品質水準: 100
• フォーム: powder
• メーカー／製品名: ATTO-TEC GmbH
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV吸収: λ: 650.0-656.0 nm A_max
• 適合性: corresponds for gel electrophoresis; suitable for fluorescence
• 保管温度: −20°C

詳細

詳細

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

アプリケーション

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

特徴および利点

Atto 647N- Streptavidin has the following benefits-

• Highly photostable. Strong absorption.
• Excellent fluorescent quantum yield.
• Highly resistant to ozone degradation.
• Minimal triplet formation.

アナリシスノート

dye-to-protein ratio ≥1.5

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)