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製品種目
BioReagent
品質水準
フォーム
powder
メーカー／製品名
ATTO-TEC GmbH
蛍光検出
λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0
適合性
suitable for fluorescence
保管温度
−20°C
アプリケーション
Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.
アナリシスノート
free of unconjugated dye
法的情報
本製品は研究専用です。商業目的で使用する場合は、ライセンス取得について知的所有権（IP）保持者（ATTO-TEC GmbH、ドイツ）にお問い合わせください。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
40709-1MG-F:
40709-1MG-BULK-F:
40709-BULK-F:
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Ronit Freeman et al.
Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed
Andrea D Lehmann et al.
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Journal of combinatorial chemistry, 11(1), 146-150 (2008-12-17)
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with this molecule are then identified and isolated for compound structure determination. Here we describe
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Testosterone (T), predominantly acting through its derivative 17β-estradiol (E2), regulates the brain's sexual differentiation in rodents during the perinatal sensitive period, which mirrors the window of vulnerability to the adverse effects of general anesthetics. The mechanisms of anesthesia's adverse effects
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資料
Fluorescent Labeling of Peptides
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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