14-484-M

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

• 別名: PRKCA, Protein kinase C alpha type
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in baculovirus infected Sf21 cells
• 製品種目: Upstate^®
• 比活性: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• シェルフライフ: 1 yr
• 分子量: M_w 78 kDa
• 精製方法: (Ni²⁺/NTA-agarose)
• 交差性: human
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• アクセッション番号: (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. ); NM_002737.2
• NCBIアクセッション番号: NM_002737.2
• UniProtアクセッション番号: P17252
• 保管温度: −70°C
• 遺伝子情報: human ... PRKCA(5578)

詳細

詳細

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC

Recombinant, full-length human PKCα, containing N-terminal His6 tag

アプリケーション

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

生物化学的／生理学的作用

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

包装

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

品質

routinely evaluated by phosphorylation of Histone H1.

ターゲットの説明

PKCα

物理的形状

Ni²⁺/NTA-agarose

保管および安定性

1 year at -70°C

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(℃): Not applicable