05-118

Anti-FGF-2/basic FGF Antibody, clone bFM-2

clone bFM-2, Upstate^®, from mouse

• 別名: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: bFM-2, monoclonal
• 化学種の反応性: mouse, bovine, human, rat
• メーカー／製品名: Upstate^®
• テクニック: immunohistochemistry: suitable; radioimmunoassay: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NM_002006.3
• UniProtアクセッション番号: P09038
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FGF2(2247) ; mouse ... Fgf2(281161) , Fgf2(14173) ; rat ... Fgf2(54250)

詳細

詳細

FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.

特異性

This antibody detects FGF-2/basic FGF.

This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.

免疫原

Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
成長因子及び受容体

Detect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.

Immunohistochemistry:
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).

品質

Evaluated on basic FGF (Catalog #01-114).

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).

ターゲットの説明

17.5 kDa

物理的形状

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.

保管および安定性

Stable for 1 year at -20ºC from date of receipt.

アナリシスノート

Control
Fetal liver or kidney tissue.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable