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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
BP53-12, monoclonal
化学種の反応性
human
包装
antibody small pack of 25 μL
メーカー／製品名
Upstate®
テクニック
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2a
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TP53(7157)
詳細
The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.
特異性
Recognizes p53.
免疫原
Recombinant human wild type p53
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
転写因子
転写因子
Anti-p53 Antibody, clone BP53-12 is a high quality Mouse Monoclonal Antibody for the detection of p53 & has been validated in IHC, IP & WB.
品質
routinely evaluated on RIPA lysate of human Raji cells
ターゲットの説明
53 kDa
関連事項
Replaces: 04-1083
物理的形状
Protein A purified
Format: Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
保管および安定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Raji cell lysate
Raji cell lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-224-25UL:
05-224-100UL:
05-224:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
The estrogen receptor alpha pathway induces oncogenic Wip1 phosphatase gene expression.
Hye-Sook Han, Eunsil Yu, Ji-Young Song, Ji-Young Park, Se Jin Jang, Jene Choi et al.
Molecular Cancer Research null
Jean-Philippe Cosse et al.
Molecular cancer, 6, 61-61 (2007-09-27)
It is more and more recognized that hypoxia plays a role in the resistance of cancer cells to chemotherapy. However, the mechanisms underlying this resistance still need deeper understanding. The aim of this study was to investigate the effect of
Differential effect of hypoxia on etoposide-induced DNA damage response and p53 regulation in different cell types.
Sermeus, Audrey, et al.
Journal of Cellular Physiology (2013)
Dan-Dan Li et al.
PloS one, 7(9), e45058-e45058 (2012-10-02)
Topotecan produces DNA damage that induces autophagy in cancer cells. In this study, sensitising topotecan to colon cancer cells with different P53 status via modulation of autophagy was examined. The DNA damage induced by topotecan treatment resulted in cytoprotective autophagy
N A Barlev et al.
Molecular cell, 8(6), 1243-1254 (2002-01-10)
Cellular DNA damage causes stabilization and activation of the tumor suppressor and transcription factor p53, in part by promoting multiple covalent modifications of the p53 protein, including acetylation. We investigated the importance of acetylation in p53 function and the mechanism
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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