コンテンツへスキップ
Merck

P5813

Sigma-Aldrich

モノクロナール抗p53 マウス宿主抗体

clone BP53-12, ascites fluid

別名:

抗TRP53抗体, 抗形質転換関連タンパク質53抗体

ログイン組織・契約価格を表示する


About This Item

MDL番号:
UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

ascites fluid

抗体製品タイプ

primary antibodies

クローン

BP53-12, monoclonal

分子量

antigen 53 kDa

含みます

15 mM sodium azide

化学種の反応性

human

テクニック

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein

アイソタイプ

IgG2a

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... TP53(7157)

類似した製品をお探しですか? 訪問 製品比較ガイド

詳細

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

免疫原

リコンビナントヒト野生型p53タンパク質。

アプリケーション

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • western blotting
  • immunohistochemistry
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

生物化学的/生理学的作用

The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

10 - Combustible liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

P5813-100UL:
P5813-.2ML:
P5813-.5ML:
P5813-BULK:
P5813-VAR:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Dominant-negative p53 mutant R248Q increases the motile and invasive activities of oral squamous cell carcinoma cells
Nakazawa S, et al.
Biomedical Research, 40(1), 37-49 (2019)
How narrow-band and broad-band uvb irradiation influences the histomorphology evaluations of experimental animals? skin-a comparative study
Borowska K
Dermatology Online Journal, 8(3e), e5-e5 (2017)
J Bártková et al.
International journal of cancer, 49(2), 196-202 (1991-09-09)
Expression of the p53 oncoprotein was examined in a wide range of primary human testicular germ-cell tumours using a new mouse monoclonal antibody (MAb) BP53-11 raised and characterized in this study, in parallel with a polyclonal rabbit antiserum CM-1. Immunohistochemistry
A p53-like protein from a freshwater mollusc Lamellidens corrianus
Mohanty BP
Indian Journal of Biochemistry & Biophysics, 121-139 (2006)
Identification and characterization of different subpopulations in a human lung adenocarcinoma cell line (A549)
Croce MV, et al.
Pathology Oncology Research, 5(3), 197-204 (1999)

資料

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら(テクニカルサービス)