P248

モノクローナル抗PARP抗体 マウス宿主抗体

clone C-2-10, ascites fluid

• 別名: モノクローナル抗ポリ[ADP-リボース]ポリメラーゼ抗体
• MDL番号: MFCD01322527
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: C-2-10, monoclonal
• 分子量: antigen 116 kDa (PARP); antigen 85 kDa (prICE/caspase-3 cleavage product)
• 化学種の反応性: hamster, rat, mouse, primate
• 以下との反応禁止: avian
• テクニック: ELISA: suitable (Assay Dependent); immunocytochemistry: suitable (Assay Dependent); immunofluorescence: suitable (Assay Dependent); immunohistochemistry: 1:10-1:100; western blot: 1:500
• アイソタイプ: IgG1
• UniProtアクセッション番号: P09874
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Parp1(11545) , Parp2(11546) ; rat ... Parp1(25591) , Parp2(290027)

詳細

詳細

Monoclonal Anti-PARP (mouse IgG1) is produced by immunizing mice with purified calf thymus poly (ADPribose) polymerase as the antigen. This antibody recognizes a 116kDa protein which corresponds to PARP and the 85kDa apoptosis induced cleavage product of prICE (proteinase resembling interleukin 1b-converting enzyme) and CPP32 (cysteine protease). It also recognizes PARP from mouse, rat, hamster and primate sources, but fails to detect avian PARP.
Poly (ADP-ribose) polymerase 1 (PARP1)/PARP is encoded by the gene mapped to human chromosome 1q42. It is a nuclear protein with molecular mass of 113kDa and is characterized with three functional domains. It belongs to the PARP superfamily.

PARP1 poly(ADP-ribose) polymerase 1) is also termed as ARTD1. It is an abundant nuclear protein that belongs to the PARP family. This gene is located on human chromosome 1q42.

特異性

エピト-プは、PARPのDNA結合ドメインのアミノ酸216-375の間に認められます。抗体は、PARP(116 kDa)およびアポト-シス誘導によるprICE(proteinase resembling interleukin 1β-converting enzyme)およびカスパ-ゼ3(CPP32システインプロテア-ゼ)の切断産物(85 kDa)と反応します。

免疫原

calf thymus PARP. The epitope is found between amino acids 216-375 in the DNA-binding domain of PARP.

アプリケーション

Monoclonal Anti-PARP (Cleaved-Asp214) antibody has been used in western blotting and immunohistochemical staining.

生物化学的／生理学的作用

PARP1 poly(ADP-ribose) polymerase 1) and its enzymatic product PAR controls cellular processes like DNA repair, chromatin remodeling, transcription and cell death. PARP1 regulates transcription. PARP1 can induce or block homologous recombination (HR).

Poly (ADP-ribose) polymerase 1 (PARP1)/PARP participates in DNA repair. It facilitates homologous recombination. PARP controls transcription by regulating chromatin structure, altering DNA methylation patterns, acting as a co-regulator of transcription factors and interacting with chromatin insulators. It also plays an important role in the protection of cardiovascular system. PARP1 is also implicated in the maintenance of genomic stability.

物理的形状

Solution in mouse ascites fluid containing 0.02% sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable