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R4775

Sigma-Aldrich

REDExtract-N-Amp PCR ReadyMix

Ready-to-use 2X PCR Master Mix with Loading Dye

Synonym(s):
Direct plant PCR master mix, Direct tissue PCR master mix, PCR master mix with loading dye
NACRES:
NA.55

Quality Level

usage

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

feature

dNTPs included
hotstart

color

red

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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This Item
R2523P1107P4600
feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart

feature

dNTPs included, hotstart: no

color

red

color

red

color

red

color

colorless

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The REDExtract-N-Amp PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.
This PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits are sufficient for one PCR amplification per extraction. However, where additional PCR reactions are required, this supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains an inert tracking dye that allows for convenient direct loading of PCR products onto agarose gels for analysis.

Application

REDExtract-N-Amp PCR Master Mix has been used:
  • for the genotyping of fixed zebrafish embryo samples
  • in polymerase chain reaction (PCR) amplification of the DNA isolated from lichen family using the REDExtract-N-Amp Plant PCR Kit
  • in the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp seed PCR kit.

REDExtract-N-Amp PCR Master Mix is also suitable for colony PCR.

Features and Benefits

  • A Gel-ready- No loading buffer or tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification. The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue).
  • Convenience- Hot-start feature in JumpStart products allows reaction set up at room temperature, which can be beneficial when working with multiple samples. Ready-mix format reduces setup time.
  • Reproducibility- Reduces the risk of contamination from multiple pipet steps and provides consistent performance across both reactions and time.
  • High Specificity & Yield- JumpStart Taq DNA polymerase, an antibody inactivated hot-start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

R4775-12ML:4.548174009468E12
R4775-1.2ML:4.548174009444E12
R4775-125ML:4.548174009451E12
R4775-.15ML:4.548174009437E12


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Ventral tegmental area dopamine cell activation during male rat sexual behavior regulates neuroplasticity and d-amphetamine cross-sensitization following sex abstinence
Beloate LN, et al.
The Journal of Neuroscience, 36, 9949-9961 (2016)
Darinka Gjorgieva et al.
BioMed research international, 2013, 276417-276417 (2013-07-19)
Heavy metals have the potential to interact and induce several stress responses in the plants; thus, effects of heavy metal stress on DNA damages and total antioxidants level in Urtica dioica leaves and stems were investigated. The samples are sampled
Rasmus H Jensen et al.
Journal of clinical microbiology, 49(2), 549-556 (2010-12-15)
Candida palmioleophila has previously been misidentified as C. famata or C. guilliermondii. We have investigated traditional and modern identification methods for the identification of this and related species. Forty-one clinical isolates previously identified as C. famata or C. guilliermondii and
Targeted mutagenesis in the progeny of maize transgenic plants.
Yang M
Plant Molecular Biology, 70(6), 669-679 (2009)
Phylogenetic placement, species delimitation, and cyanobiont identity of endangered aquatic Peltigera species (lichen-forming Ascomycota, Lecanoromycetes).
Miadlikowska J
American Journal of Botany, 101(7), 1141-1156 (2014)

Articles

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Protocols

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service