TRI Reagent has been used:
- in the isolation of total RNA from various tissue samples and cells 
- for the lysis of monocytes 
- to isolate cytoplasmic RNA from SARS-CoV infected Vero cells 
- to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus 
- to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine 
The RNA, DNA and protein that are isolated using TRI reagent®
can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro
translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.