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Betaine solution

5 M, PCR Reagent

Linear Formula:
CAS Number:
Molecular Weight:
MDL number:
PubChem Substance ID:

Quality Level


PCR Reagent




vial of 1.5 mL


5 M


PCR: suitable





foreign activity

DNase, RNase, none detected

storage temp.


SMILES string




InChI key


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General description

Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.


Betaine solution has been used as a component of
  • loop-mediated isothermal amplification (LAMP) reactions
  • polymerase chain reaction for genomic DNA amplification
  • quantitative polymerase chain reaction (qPCR) for telomere length analysis
  • in the PCR amplification of CGG repeats in the genomic DNA.


1.5mL per vial

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Hela Azaiez et al.
Human mutation, 24(4), 305-311 (2004-09-15)
Genetic testing was completed on 1,294 persons with deafness referred to the Molecular Otolaryngology Research Laboratories to establish a diagnosis of DFNB1. Exon 2 of GJB2 was screened for coding sequence allele variants by denaturing high-performance liquid chromatography (DHPLC) complemented
Eniko Sajti et al.
Nature immunology, 21(2), 221-231 (2020-01-22)
The lung is inhabited by resident alveolar and interstitial macrophages as well as monocytic cells that survey lung tissues. Each cell type plays distinct functional roles under homeostatic and inflammatory conditions, but mechanisms establishing their molecular identities and functional potential
Diet quality and telomere length in older Australian men and women
Milte CM, et al.
European Journal of Nutrition, 57(1), 363-372 (2018)
P N Hengen
Trends in biochemical sciences, 22(6), 225-226 (1997-06-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. This month's column discusses the use of additives for optimizing the amount and quality of product obtained through multiplex and
Betaine chemistry, roles, and potential use in liver disease
Day CR and Kempson SA
Biochimica et biophysica acta. General subjects, 1860(6), 1098-1106 (2016)


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