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XNAT

Sigma-Aldrich

REDExtract-N-Amp Tissue PCR Kit

sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

EC Number:
NACRES:
NA.55

Quality Level

usage

sufficient for 10 reactions
10 reactions sufficient for 10 amplifications
10 reactions sufficient for 10 extractions
sufficient for 100 reactions
100 reactions sufficient for 100 amplifications
100 reactions sufficient for 100 extractions
sufficient for 1000 reactions
1000 reactions sufficient for 1000 amplifications
1000 reactions sufficient for 1000 extractions

feature

dNTPs included
hotstart

color

red

shipped in

wet ice

storage temp.

−20°C

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General description

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA. The kit includes a PCR ReadyMix, specially formulated for direct PCR amplification from the extract. This formulation uses an antibody-based hot start, for specific amplification. The REDExtract-N-Amp Tissue PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp tissue PCR kit has been used for DNA extraction and in polymerase chain reaction (PCR).

Features and Benefits

  • Perfect for quick genomic DNA isolation for genotyping
  • Single-step extraction of genomic DNA for PCR in 15 minutes
  • PCR ReadyMix, specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4oC for at least 6 months
  • REDExtract-N-Amp contains a tracking dye for convenient and direct loading of PCR reactions onto agarose gels for analysis

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Principle

The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. How do I quantitate my DNA with XNAT, REDExtract-N-Amp® Tissue PCR Kit?

    The DNA extract generated using the Extract-N-Amp kit is very crude, and it is not possible to obtain a reliable 260/280 ratio.  For quantitation of pure DNA, we recommend using one of our GenElute genomic DNA kits.

  2. How do I store my DNA samples for use with XNAT, REDExtract-N-Amp®  Tissue PCR Kit?

    DNA samples can be stored for up to 6 months at 2-8°C with the tissue sample removed.

  3. Are the XNAT, REDExtract-N-Amp® Tissue PCR Kit components available separately?

    Only R4775, the REDExtract-N-Amp PCR Reaction Mix is available separately.

  4. What is the shelf life of the kit XNAT, REDExtract-N-Amp® Tissue PCR Kit and how should I store it?

    The shelf life of XNAT has not been determined.  However, based on our storage guidelines, it should be usable for 1 year from the date of receipt. The REDExtract-N-Amp Tissue PCR Kit can be stored at 2-8°C for up to three weeks. For long-term storage, greater than three weeks, -20°C is recommended. Do not store in a "frost-free" freezer.  We recommend to store in 10 reaction aliquots.

  5. I am unable to detect any PCR product with Product XNAT, REDExtract-N-Amp® Tissue PCR Kit.

    From XNAT Technical Bulletin, troubleshooting guide table:  PCR reaction may be inhibited due to contaminants in the tissue extract.  Dilute the tissue extract with a 50:50 mix of extraction and neutralization solutions.  To test for inhibition, include a DNA control and/or spike a known amount of template (100-500 copies) into the PCR along with the tissue extract.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Articles

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

Extract-N-Amp™ FFPE Tissue PCR Protocol

Extract-N-Amp FFPE Tissue PCR Protocol

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

he REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service