R2523

REDTaq^® ReadyMix^™ PCR Reaction Mix

Complete PCR reagent with standard Taq DNA Polymerase and inert dye

• Synonym(s): PCR master mix, PCR master mix with loading dye, Taq PCR master mix
• NACRES: NA.55

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 100 reactions; sufficient for 20 reactions
• feature: dNTPs included: no; hotstart: no
• technique(s): PCR: suitable
• color: red
• input: purified DNA
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

REDTaq^® ReadyMix^™ is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. The red loading dye migrates at approximately the same rate as a 125 base pair fragment in a 1% agarose gel. The inert dye has no effect on the amplification process, and therefore, a sample can be easily re-amplified such as in “nested PCR”.

Application

REDTaq^® ReadyMix^™ PCR Reaction Mix has been used:

• for routine polymerase chain reaction (PCR) amplification.[1][2][3][4]
• for the PCR amplification of cDNA[5]
• in semi-quantitative PCR[6]

Features and Benefits

The benefits of REDTaq^® with the convenience of ReadyMix^™

• Saves preparation time
• Reduces risk of contamination from multiple pipet steps and and increased reproducibility.
• Provides consistent reaction-to-reaction performance
• No loading buffer/tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification
• The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue)
• Amplifies targets up to 7 kb in length
• Because no loading buffer has been added, a sample can be easily re-amplifed such as in nested PCR.
• Stable at 4°C or −20°C.
• Ready-mix format reduces setup time.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL

Preparation Note

Add 25 μl of ReadyMix to template, primers and water to a final reaction volume of 50 μl. The PCR product is easily separated from the dye by standard purification methodologies. The inert red dye has no effect on automated sequencing and is suitable for restriction enzyme digestion, ligation and other downstream manipulations.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1