expressed in E. coli
sufficient for 10000 reactions
sufficient for 3000 reactions
sufficient for 500 reactions
dNTPs included: no
suitable for PCR and automated sequencing reactions
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12 - Non Combustible Liquids
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.