Dimethyl sulfoxide

for molecular biology

Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO, Methyl Sulfoxide
Linear Formula:
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


for molecular biology

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line






autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F


DNA sequencing: suitable
PCR: suitable
transfection: suitable


≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)



refractive index

n20/D 1.479 (lit.)


189 °C (lit.)


16-19 °C (lit.)


H2O: miscible (completely)


1.10 g/mL (lit.)


passes test


suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.


DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.


1 L in glass bottle
50, 100, 250, 500 mL in glass bottle

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.


Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.


10 - Combustible liquids

WGK Germany


Flash Point F

188.6 °F - closed cup

Flash Point C

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  4. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  5. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. Is Product D8418, Dimethyl sulfoxide, assayed for DNase, RNase and protease?

    Yes. It is a molecular biology grade product, and it is tested for DNase, RNase and protease. For a lot to pass none of those enzymes may be detected.

  8. Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?

    Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.

  9. With what materials is Product D8418, Dimethyl sulfoxide, compatible?

    The compatibility of DMSO with various materials is listed here: Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, PTFE (Teflon) and FEP. Moderately compatible: polystyrene, ECTFE/ETFE. Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate.

  10. DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?

    The procedure to freeze cells is in the product information sheet (under Documents, above).

Petra Smatlikova et al.
Neuro-degenerative diseases, 19(1), 22-34 (2019-06-06)
Huntington's disease (HD) is a devastating neurodegenerative disorder caused by CAG triplet expansions in the huntingtin gene. Oxidative stress is linked to HD pathology, although it is not clear whether this is an effect or a mediator of disease. The...
Hongjie An et al.
Advances in colloid and interface science, 222, 9-17 (2014-08-17)
The observation by Atomic Force Microscopy of a range of nanophases on hydrophobic surfaces poses some challenging questions, not only related to the stability of these objects but also regarding their wetting properties. Spherical capped nanobubbles are observed to exhibit...
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many...
Claudia C Faria et al.
Cancer research, 75(1), 134-146 (2014-11-14)
Medulloblastoma is the most common malignant pediatric brain tumor, with metastases present at diagnosis conferring a poor prognosis. Mechanisms of dissemination are poorly understood and metastatic lesions are genetically divergent from the matched primary tumor. Effective and less toxic therapies...
Mulmudi Hemant Kumar et al.
Advanced materials (Deerfield Beach, Fla.), 26(41), 7122-7127 (2014-09-13)
Lead free perovskite solar cells based on a CsSnI3 light absorber with a spectral response from 950 nm is demonstrated. The high photocurrents noted in the system are a consequence of SnF2 addition which reduces defect concentrations and hence the...
Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service