D1435

Dimethyl sulfoxide

meets EP testing specifications, meets USP testing specifications

• Synonym(s): DMSO
• Linear Formula: (CH₃)₂SO
• CAS Number: 67-68-5
• Molecular Weight: 78.13
• Beilstein: 506008
• EC Number: 200-664-3
• MDL number: MFCD00002089
• PubChem Substance ID: 57654071
• NACRES: NA.71

Properties

• Agency: meets EP testing specifications; meets USP testing specifications
• Quality Level: 200
• vapor density: 2.7 (vs air)
• vapor pressure: 0.42 mmHg ( 20 °C)
• Assay: ≥99.5%
• form: liquid
• autoignition temp.: 573 °F
• expl. lim.: 42 %, 63 °F
• technique(s): cryopreservation: suitable
• impurities: ≤0.1% water (Karl Fischer, USP); ≤0.2% water (Karl Fischer, EP)
• color: colorless
• refractive index: n20/D 1.478-1.479; n20/D 1.479 (lit.)
• bp: 189 °C (lit.)
• mp: 16-19 °C (lit.)
• solubility: H₂O: miscible (completely)
• density: 1.100-1.104 g/mL at 25 °C (relative density (EP)); 1.10 g/mL (lit.)
• UV absorption: λ: 285 nm A_max: ≤0.20; λ: 295 nm A_max: ≤0.20
• SMILES string: CS(C)=O
• InChI: 1S/C2H6OS/c1-4(2)3/h1-2H3
• InChI key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

Application

Dimethyl sulfoxide has been used:

• for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments
• as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells
• for the suspension and separation of buccal cells
• a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Biochem/physiol Actions

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant. It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves