Merck
All Photos(1)

E3004

Sigma-Aldrich

Extract-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

Synonym(s):
Tissue direct PCR master mix, Plant direct PCR master mix
NACRES:
NA.55

Quality Level

usage

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

feature

dNTPs included
hotstart

color

colorless

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

Extract-N-Amp PCR ReadyMix is a specially formulated hot start PCR master mix for direct PCR amplification from the extract.
PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, if additional PCR reactions are required, supplemental PCR ReadyMix may be needed.

Application

Extract-N-Amp PCR ReadyMix has been used for the following applications:
  • Genomic DNA extraction
  • Fungal DNA extraction and amplification by polymerase chain reaction (PCR) reactions
  • Direct PCR amplification
  • Genotyping
  • Real-Time PCR

Packaging

12, 125 mL in poly bottle

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service