XNAPS

REDExtract-N-Amp^™ Plant PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

• Synonym(s): Plant direct PCR kit, Plant direct PCR kit with loading dye
• UNSPSC Code: 41106303
• NACRES: NA.55

Properties

• usage: sufficient for 10 amplifications; sufficient for 10 extractions; sufficient for 10 reactions
• feature: dNTPs included; hotstart
• technique(s): PCR: suitable
• color: red
• storage temp.: −20°C

Description

General description

The REDExtract-N-Amp^™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix^™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp^™ Plant PCR Kit has been used to:

• extract genomic DNA from mycelial lawns and leaves
• screen leaf tissues from putative transgenic potato plants
• perform polymerase chain reaction (PCR)

Features and Benefits

• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• REDExtract-N-Amp^™ requires no loading buffers or tracking dyes required for gel analysis
• Compatible with high-throughput requirements for genetic analysis of plants
• Extract stable at 4 °C for at least 6 months

Components

Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H315,H317,H319,H411
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids