Merck
All Photos(6)

G2N70

Sigma-Aldrich

GenElute Plant Genomic DNA Miniprep Kit

greener alternative

sufficient for 70 purifications

Synonym(s):
Plant Genomic DNA Miniprep, Gen Elute
NACRES:
NA.55

Quality Level

usage

sufficient for 70 purifications

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

storage temp.

15-25°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.

Application

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots

GenElute Plant Genomic DNA Miniprep Kit has been used:
  • in the purification of genomic DNA isolated from neem tissues
  • to extract genomic DNA from embryos
  • to isolate DNA from plant samples

Features and Benefits

  • Starting material: Up to 100 mg of plant tissue
  • Expected yield: Up to 20 μg
  • Elution volume: 100 - 200 μl
  • Time required: < 40 min
  • RNase treatment required: No

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation Solution

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Articles

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service