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Merck

H3917

Sigma-Aldrich

肝素酶 I 和 III 混合物 来源于肝素黄杆菌

lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)

别名:

Heparinase I and Heparinase III blend

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Flavobacterium heparinum)

品質等級

共軛

conjugate (Glucosaminoglycan)

形狀

lyophilized powder

比活性

≥200 units/mg protein

濃度

≥200 unit/mg protein (enzyme + BSA)

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

肝素酶是一种可诱导的、非细胞外的肝素降解酶。三种肝素化是 肝素黄杆菌 产生的 ,含有特定的肝素序列。

應用

来自肝素黄杆菌的肝素酶I和II混合物已被用于:
  • 消化绵羊玻璃体的硫酸乙酰肝素
  • 人胚胎肾细胞
  • 来自动脉组织的糖胺聚糖
  • P0 视网膜消化

生化/生理作用

肝素酶 I 和 III 在多种生物学过程中发挥重要作用:调节细胞生长因子相互作用、细胞-脂蛋白相互作用、新生血管形成。 在 2-O-硫酸化 α 存在下裂解高硫酸化多糖链-L-吡喃二糖糖醛酸和 β-多糖的 D-吡喃葡萄糖醛酸残基。
肝素降解裂解酶,识别硫酸肝素蛋白聚糖为其主要底物。

包裝

按肝素酶 I 单位销售

單位定義

在 25℃、PH 7.5 下,以肝素、钠作为肝素酶 I 的底物,每小时 1 个单位将形成 0.1 μmol 不饱和糖醛酸。

在 25℃、PH 7.5 下,采用牛肾肝素,硫酸盐作为肝素酶 III 的底物,每小时 1 个单位将形成 0.1 μmol 不饱和糖醛酸。

25°C、pH 7.5 下 1 个单位不饱和糖醛酸形成(0.1 μmol/小时)。1 个国际单位 (I.U.) 相当于约 600σ 单位。包装尺寸以 σ 单位销售。

其他說明

Hep I 和 4.2.2.8 III 型肝炎

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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S Ernst et al.
Critical reviews in biochemistry and molecular biology, 30(5), 387-444 (1995-01-01)
Glycosaminoglycans (GAGs) play an intricate role in the extracellular matrix (ECM), not only as soluble components and polyelectrolytes, but also by specific interactions with growth factors and other transient components of the ECM. Modifications of GAG chains, such as isomerization
Farizeh Aalam et al.
PLoS pathogens, 16(10), e1008968-e1008968 (2020-10-20)
Despite 25 years of research, the basic virology of Kaposi Sarcoma Herpesviruses (KSHV) in B lymphocytes remains poorly understood. This study seeks to fill critical gaps in our understanding by characterizing the B lymphocyte lineage-specific tropism of KSHV. Here, we
P M Galliher et al.
Applied and environmental microbiology, 41(2), 360-365 (1981-02-01)
Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter
D A Chappell et al.
The Journal of biological chemistry, 268(19), 14168-14175 (1993-07-05)
Bovine milk lipoprotein lipase (LPL) induced binding, uptake, and degradation of 125I-labeled normal human triglyceride-rich lipoproteins by cultured mutant fibroblasts lacking LDL receptors. The induction was dose-dependent and occurred whether LPL and 125I-lipoproteins were added to incubation media simultaneously or
Cassandra R Blanchette et al.
PLoS genetics, 13(1), e1006525-e1006525 (2017-01-10)
The regulation of cell migration is essential to animal development and physiology. Heparan sulfate proteoglycans shape the interactions of morphogens and guidance cues with their respective receptors to elicit appropriate cellular responses. Heparan sulfate proteoglycans consist of a protein core

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