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H6512

Sigma-Aldrich

肝素酶II 来源于肝素黄杆菌

Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA)

别名:

肝素裂解酶II

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About This Item

CAS号:
149371-12-0
MDL號碼:
MFCD00131319
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Flavobacterium heparinum)

品質等級

200

共軛

conjugate (Glucosaminoglycan)

形狀

lyophilized powder

比活性

≥100 units/mg protein (enzyme + BSA)

分子量

84.1 kDa

儲存溫度

−20°C

一般說明

肝素酶 II 是肝素黄杆菌（Flavobacterium heparinum，又称肝素土地杆菌1）的三大肝素降解酶之一。它属于多糖裂解酶家族PL21。

應用

来自肝素黄杆菌(Flavobacterium heparinum)的肝素酶 II 已用于：
  • 在外泌体分离过程中消化硫酸肝素
  • 在脊索细胞条件培养基 (NCCM) 中消化硫酸肝素以研究 NCCM 中糖胺聚糖的含量
  • 用作细胞表面聚糖加工过程中消化缓冲液的组分
  • 用作肝素裂解酶(HSase)混合物，去除293ͰT/ACE2细胞表面的硫酸肝素并研究组蛋白对假病毒感染性的影响

生化／生理作用

肝素酶 II 可在己糖胺和糖醛酸残基（葡萄糖醛酸和艾杜糖醛酸）之间的α (1-4)键处裂解切割硫酸乙酰肝素，并可控制肝素裂解（相对活性约为 2:1），主要产生二糖。在三种肝素酶中，肝素酶 II 具有最广泛的底物特异性肝素酶有助于破译底物的复杂结构。肝素干扰 PCR和RNA逆转录中的DNA转录。肝素酶 II 已用于去除肝素，以进行基因组 DNA 下游分析。

單位定義

在25 °C、pH 7.0条件下，一个单位每小时可形成0.1 μM不饱和糖醛酸。 一个国际单位（I.U.）相当于大约600个Sigma单位。

其他說明

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Moffat, C.F., et al.
Proc. 8th Int. Symp. Glycoconjugates, 1, 79-79 (1985)
Judith Mary Reyes Ballista et al.
Frontiers in cell and developmental biology, 11, 1085913-1085913 (2023-02-07)
Chikungunya virus (CHIKV) is the causative agent of the human disease chikungunya fever, characterized by debilitating acute and chronic arthralgia. No licensed vaccines or antivirals are currently available for CHIKV. Therefore, the prevention of attachment of viral particles to host
McLean, M.W., et al.
Proc. 8th Int. Symp. Glycoconjugates, 1, 73-73 (1985)
David Shaya et al.
The Journal of biological chemistry, 285(26), 20051-20061 (2010-04-21)
Heparinase II (HepII) is an 85-kDa dimeric enzyme that depolymerizes both heparin and heparan sulfate glycosaminoglycans through a beta-elimination mechanism. Recently, we determined the crystal structure of HepII from Pedobacter heparinus (previously known as Flavobacterium heparinum) in complex with a
R F Sewell et al.
The Biochemical journal, 264(3), 777-783 (1989-12-15)
Xyloside-initiated 35SO4(2-)-labelled glycosaminoglycans were isolated from the medium of cultured bovine glomeruli and covalently coupled to Sepharose 4B to construct a solid-phase substrate suitable for the detection of endoglycosidases. The substrate is rendered specific for heparitinase by prior digestion with
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