推荐产品
生物源
bacterial (Proteus vulgaris)
品質等級
共軛
(Glucosaminoglycan)
形狀
lyophilized powder
比活性
0.3-3 units/mg solid
分子量
~120 kDa by gel filtration
成份
Protein, ~35% biuret
應用
diagnostic assay manufacturing
儲存溫度
−20°C
正在寻找类似产品? 访问 产品对比指南
應用
Sigma的软骨素酶ABC被用来消化O连接的硫酸软骨素类糖胺聚糖(CSGAG)。该酶还被用来消化蛋白质多糖中的糖胺聚糖链，在哺乳动物系统中构建和表达重组的多功能蛋白聚糖基因。此外，它还被用来消化蛋白聚糖复合物中的硫酸软骨素。该酶还被用于从蛋白聚糖中去除糖链，从而消除蛋白聚糖的轴突生长抑制作用。
生化／生理作用
软骨苷酶ABC催化含有(1-4)-β-D-己糖氨基和(1-3)-β-D-葡萄糖醛酸基(或(1-3)-α-L-iduronosyl)的多糖与含有4-脱氧-β-D-葡萄糖-4-enuronosyl基团的双糖的清除降解。它作用于硫酸软骨素4、硫酸软骨素6、硫酸皮肤素。但是，它对透明质酸盐作用缓慢。其分子量约为120 kDa，含分子量分别为86 kDa和32 kDa的两个不同亚单位。硫酸软骨素pH值为8.0，透明质酸pH值为6.8。最佳温度为37℃。1mM Zn2+ 可作为其抑制剂，0.05 M乙酸为该酶的激活剂。
包裝
包装按照软骨素酶C活性。
單位定義
在37°C、pH 8.0条件下，一单位每分钟将释放1.0 μmole的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖)-4-烯-吡喃糖醛糖醛酸）-4-O-磺基-D-半乳糖的混合物和1.0 μmole来自鲨鱼软骨硫酸软骨素的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖-4-烯-吡喃糖醛糖醛酸)-6-O-磺基-D-半乳糖。
外觀
含 Tris 缓冲盐的冻干粉
準備報告
在0.01%牛血清白蛋白水溶液(BSA)中复溶。随后的稀释液可以制成pH 8.0的含有50mm Tris,60mm乙酸钠和0.02% BSA的缓冲液。溶液需新鲜配制。
其他說明
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
G A de Munk et al.
The Biochemical journal, 290 ( Pt 3), 655-659 (1993-03-15)
Thrombomodulin (TM), a membrane proteoglycan on endothelial cells, binds thrombin in a 1:1 complex, accelerates the protein C activation by thrombin, promotes the thrombin inactivation by antithrombin III and inhibits the procoagulant properties of thrombin. The inactivation of single-chain urokinase-type
Y Zhang et al.
The Journal of biological chemistry, 273(33), 21342-21351 (1998-08-08)
Versican is a member of the large aggregating chondroitin sulfate proteoglycan family. We have expressed in NIH3T3 fibroblasts a recombinant versican mini-gene comprising the G1 and G3 domains and 15% of the CS domain. We observed that expression of the
Mafalda Resende et al.
International journal for parasitology, 39(11), 1195-1204 (2009-03-28)
Individuals living in areas with high Plasmodium falciparum transmission acquire immunity to malaria over time and adults have a markedly reduced risk of contracting severe disease. However, pregnant women constitute an important exception. Pregnancy-associated malaria is a major cause of
Nicolett Kanyo et al.
Scientific reports, 10(1), 22422-22422 (2021-01-01)
The glycocalyx is thought to perform a potent, but not yet defined function in cellular adhesion and signaling. Since 95% of cancer cells have altered glycocalyx structure, this role can be especially important in cancer development and metastasis. The glycocalyx
Francisca Yosaatmadja et al.
Malaria journal, 7, 51-51 (2008-03-28)
Malaria in pregnancy is characterized by accumulation of infected erythrocytes (IE) in the placenta. The key ligand identified as mediating this process is a Plasmodium falciparum erythrocyte membrane protein 1 family member, termed VAR2CSA. VAR2CSA appears to be the main
商品
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门