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D4184

Sigma-Aldrich

JumpStart™ Taq DNA聚合酶

without MgCl2

别名:

hot start DNA polymerase, hot start PCR

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About This Item

MDL號碼:
MFCD00166026
分類程式碼代碼:
12352204
NACRES:
NA.55

品質等級

300

形狀

liquid

用途

sufficient for 1500 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions

特點

dNTPs included: no
hotstart

濃度

2.5 units/μL

技術

PCR: suitable

顏色

colorless

輸入

purified DNA

適合性

suitable for PCR

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

Taq DNA聚合酶活性通过将该酶与JumpStart™ Taq抗体（Taq DNA聚合酶的中和性单克隆抗体）结合而失活。抗体失活为热启动聚合酶链反应(PCR)提供了一种简单、有效的方法。Hot start PCR通过减少非特异性扩增产物和引物-二聚物产物的产生，可以显著提高DNA扩增结果。当用于PCR时，JumpStart Taq DNA聚合酶在低温（室温）下是不活跃的。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全激活。

應用

JumpStart™ Taq DNA聚合酶已用于：
  • PCR扩增从植物标本中分离的DNA。
  • PCR反应混合物，进行随机扩增的多态性DNA聚合酶链式反应(RAPD PCR)。
  • 使用特定癌细胞系扩增和检测EGFR外显子19中的点突变。
  • 实时荧光定量PCR
  • 用于复杂基因组或cDNA模板的PCR扩增
  • 用于极低拷贝数靶标的PCR扩增
  • 用于多个热循环（>35次）的PCR扩增，且多个引物对在同一反应管中
  • 用于需要降低非特异性扩增的PCR扩增
  • 用于多重PCR
  • 用于降低引物二聚体

特點和優勢

  • 降低非特异性扩增
  • 提高PCR特异性及产量
  • 减少与手动或蜡热启动方法相关的设置时间担忧
  • 低于1分钟的激活时间

包裝

JumpStart Taq DNA聚合酶是以10x反应缓冲液形式提供的，并可选择是否含有MgCl2。 不含镁的10x缓冲液还包含单独一管的25 mM MgCl2用于优化。
按不含 MgCl2的10x反应缓冲液形式提供。 包含单独一管的25 mM MgCl2

其他說明

Sigma的JumpStart Taq DNA聚合酶是一种抗体失活的热启动酶，设计用于最大限度降低非特异性扩增并同时提高靶标的产量。 一旦反应温度达到 70°C，Taq DNA聚合酶活性就会得到恢复，使得PCR具有更高的特异性和产量。这种抗体-酶复合物可实现简单便捷的设置，并相对于手动热启动技术具有更低的污染风险。 该酶还可以被加入到预混液制备中，从而实现反应之间更高的一致性。
可访问www.sigma-aldrich.com/hotstart查看更多关于JumpStart Taq酶的详细信息。

單位定義

在74 °C，一单位的酶可以在30分钟内将10 nmol的总dNTP插入至可酸沉淀的DNA中。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的美国境外专利声明保护：US 8,404,464及US 7,972,828. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。

抗体授权用于在美国专利5,338,671和5,587,287及其在其他国家相应专利保护下的体外研究用途。
JumpStart is a trademark of Sigma-Aldrich Co. LLC

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儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

实验方案

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

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