推荐产品
品質等級
用途
sufficient for 250 reactions
特點
dNTPs included
hotstart
技術
RT-qPCR: suitable
顏色
colorless
輸入
purified RNA
相容性
ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
檢測方法
SYBR® Green
運輸包裝
wet ice
儲存溫度
−20°C
相关类别
一般說明
應用
- 检测诺如病毒基因组 I 和 II 的特定基因簇
- 定量测定基孔肯雅(CHIKV)RNA检测 mRNA 表达水平
- 扩增从人脐静脉内皮细胞 (HUVEC)和 前列腺癌细胞中提取的总 RNA
特點和優勢
- 该预混液可确保反应间的一致性和可重现性
- 缩短了制备时间,并降低了多个移液步骤造成的污染风险
- 与手动或热启动(Hot Start)方法相比,减少了设置时间
- JumpStart™Taq聚合酶可减少引物二聚体和非特异性产物形成。
- SYBR® Green I染料价格便宜、易于使用、灵敏度高
- 广泛的仪器兼容性
- 包括一个单独的ROX参考染料瓶,用于反应标准化
包裝
法律資訊
仅试剂盒组分
- SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25
訊號詞
Danger
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
商品
Sigma’s RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
实验方案
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.
Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.
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