跳转至内容
Merck
所有图片(1)

主要文件

查看所有文档

M8787

Sigma-Aldrich

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

别名:

PCR optimization

登录查看公司和协议定价
所有图片(1)

About This Item

线性分子式:
MgCl2
CAS号:
7786-30-3
分子量:
95.21
MDL號碼:
MFCD00011106
分類程式碼代碼:
12352302
PubChem物質ID:
57654422
NACRES:
NA.52
等級:
PCR Reagent
形狀:
liquid

等級

PCR Reagent

品質等級

200
300

形狀

liquid

包裝

vial of 1.5 mL

濃度

25 mM±1 mM

技術

PCR: suitable

顏色

colorless

應用

agriculture
diagnostic assay manufacturing

異物活動

DNase, RNase, none detected

儲存溫度

2-8°C

SMILES 字串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI 密鑰

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

正在寻找类似产品? 访问 产品对比指南

相关类别

一般說明

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加，镁缺乏会导致产量降低。

應用

氯化镁溶液已用作以下材料的组分：

  • 聚合酶链式反应（PCR）扩增混合液
  • 简单序列重复(ISSR)-PCR扩增混合液
  • 16S-23S rRNA基因内转录间隔区（ITS）PCR扩增及限制性内切分析（ITS-PCR- rflp）反应缓冲液
  • 用于定量逆转录-聚合酶链反应（qRT PCR）的PCR反应混合物，以扩增逆转录cDNA
  • 用于RT-PCR检测基因表达的反应混合物

適合性

适用于聚合酶链反应的优化。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCK6176
SLCH7551
SLCC7461
SLBZ9143
SLBQ4119V

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Manal A Farg et al.
Human molecular genetics, 23(13), 3579-3595 (2014-02-20)
Intronic expansion of a hexanucleotide GGGGCC repeat in the chromosome 9 open reading frame 72 (C9ORF72) gene is the major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. However, the cellular function of the C9ORF72 protein remains unknown.
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Takeshi Tomita et al.
PloS one, 9(9), e108957-e108957 (2014-10-01)
Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins
查看所有相关文献

商品

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

实验方案

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门