跳转至内容
Merck
所有图片(1)

Key Documents

更多文件

M1302

Sigma-Aldrich

M-MLV 逆转录酶

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

别名:

莫洛尼鼠白血病病毒逆转录酶

登录查看公司和协议定价
所有图片(1)

About This Item

CAS号:
9068-38-6
MDL號碼:
MFCD00283754
分類程式碼代碼:
12352202
NACRES:
NA.55

生物源

Porcine intestinal mucosa

重組細胞

expressed in E. coli

形狀

liquid

用途

sufficient for 200 reactions
 sufficient for 250 reactions

特點

dNTPs included: no
hotstart: no

濃度

200 units/μL

技術

RT-PCR: suitable

顏色

colorless

輸入

purified RNA

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。

應用

M-MLV逆转录酶已用于：
  • 制备cDNA文库或合成第一条cDNA链
  • 2步法RT-PCR检测
  • 实时定量聚合酶链反应(RT-qPCR)
  • 逆转录

特點和優勢

  • 热稳定性逆转录酶在37℃时活性佳。
  • 可用于制备长达7 kb的第一链cDNA。

包裝

本品为含DTT 的10× M-MLV逆转录酶缓冲液。

單位定義

一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。

準備報告

该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。

法律資訊

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
G F Gerard et al.
DNA (Mary Ann Liebert, Inc.), 5(4), 271-279 (1986-08-01)
We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region
查看所有相关文献

商品

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门