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M1302

Sigma-Aldrich

M-MLV 逆转录酶

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

别名:

莫洛尼鼠白血病病毒逆转录酶

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About This Item

CAS号:
9068-38-6
MDL號碼:
MFCD00283754
分類程式碼代碼:
12352202
NACRES:
NA.55

生物源

Porcine intestinal mucosa

品質等級

200

重組細胞

expressed in E. coli

形狀

liquid

用途

sufficient for 200 reactions
 sufficient for 250 reactions

特點

dNTPs included: no
hotstart: no

濃度

200 units/μL

技術

RT-PCR: suitable

顏色

colorless

輸入

purified RNA

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。

應用

M-MLV逆转录酶已用于：
  • 制备cDNA文库或合成第一条cDNA链
  • 2步法RT-PCR检测
  • 实时定量聚合酶链反应(RT-qPCR)
  • 逆转录

特點和優勢

  • 热稳定性逆转录酶在37℃时活性佳。
  • 可用于制备长达7 kb的第一链cDNA。

包裝

本品为含DTT 的10× M-MLV逆转录酶缓冲液。

單位定義

一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。

準備報告

该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。

法律資訊

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

抑制劑

产品编号
说明
价格

N8878

萘啶酸, ≥98%

N4382

萘啶酸 钠盐, powder

相關產品

产品编号
说明
价格

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
<BIG><BIG>Guzik KA, et al.</BIG></BIG>
Acta Neurobiologiae Experimentalis, 71, 193-207 (2011)
Status epilepticus evokes prolonged increase in the expression of CCL3 and CCL4 mRNA and protein in the rat brain
Guzik-Kornacka A, et al.
Acta Neurobiologiae Experimentalis, 71(2), 193-207 (2011)
D S Howland et al.
Brain research. Molecular brain research, 11(3-4), 345-353 (1991-10-11)
The phosphoprotein synapsin I is expressed exclusively in neuronal cells. We are interested in elucidating the promoter sequences involved in cell type-specific expression of the synapsin I gene. The PC12 cell line expresses the 3.4 kb and 4.5 kb synapsin
Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana
Dikaios I, et al.
Photosynthesis Research, 1-16 (2019)
G F Gerard et al.
DNA (Mary Ann Liebert, Inc.), 5(4), 271-279 (1986-08-01)
We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region
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