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一般說明
1步法： 同一管中的eAMV™ RT逆转录酶和AccuTaq LA DNA聚合酶相继发挥作用，首先生成cDNA，紧接着进行PCR扩增反应。 用于灵敏、快速的RNA分析。
2步法： 每次反应都经过单独优化，可在高保真前提下获得更高产率。适合需要更多扩增的实验，或高产率比便利性更加重要的实验。
應用
特點和優勢
- 该反转录酶可制备更长的转录本，生成长达14.1kb的cDNA。
- 低丰度mRNA检测灵敏度更高。eAMV™ RT能够成功转录其他反转录酶无法检测到的微量RNA。
- 针对复杂的二级结构的RNA,拥有超强的转录能力。
- JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。
其他說明
法律資訊
危險聲明
防範說明
危險分類
Aquatic Chronic 3
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
商品
One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.
实验方案
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA
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