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形狀
liquid
用途
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
特點
dNTPs included
hotstart
儲存條件
protect from light
技術
qPCR: suitable
顏色
colorless
輸入
purified DNA
相容性
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MiniOpticon
for use with Bio-Rad MyiQ
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
檢測方法
SYBR® Green
運輸包裝
dry ice
儲存溫度
−20°C
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一般說明
高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要,因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前,处于抗体介导的失活状态。
應用
- 基因表达
- DNA定量
- 染色质免疫沉淀(CHiP)
特點和優勢
- 只需33分钟即可获得分析结果
- 高效、灵敏的实时PCR结果
- 几乎不需要多少优化,甚至无需优化
成分
包装:
250次反应* = 2 X 1.25 mL试管
1250次反应* = 10 X 1.25 mL试管
5000次反应* = 1 X 50 mL试管
*设一次反应需20uL
其他說明
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存,可稳定存放1年。为方便使用,也可在+2至+8°C下储存长达6周。解冻后,充分混匀后再使用。不建议反复冻融产品。不过,冻融20次或+20°C存放2个月后,产品没有出现任何性能损失。
不同的实时PCR系统采用不同策略来校准荧光信号和校正孔间光学变化。 为特定仪器匹配合适的qPCR试剂至关重要。 KiCqStart SYBR Green qPCR ReadyMix不含内参染料。 兼容仪器:
- Bio-Rad® CFX384™
- Bio-Rad CFX96™
- Bio-Rad MiniOpticon™
- Bio-Rad/MJ Chromo4™
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Opticon™
- Cepheid SmartCycler®
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Illumina Eco qPCR
- Qiagen/Corbett Rotor-Gene® 3000
- Qiagen/Corbett Rotor-Gene 6000
- Qiagen/Corbett Rotor-Gene Q
- Roche LightCycler® 480
法律資訊
推薦
相關產品
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
商品
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
实验方案
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
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