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KCQS02

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix预混液™

with ROX™ for ABI instruments

别名:

qPCR master mix, sybr green qPCR

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 1250 reactions
 sufficient for 250 reactions
 sufficient for 5000 reactions

特點

dNTPs included
hotstart

儲存條件

protect from light

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

相容性

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7700
for use with ABI 7900 HT Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus

檢測方法

SYBR® Green

運輸包裝

dry ice

儲存溫度

−20°C

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相关类别

一般說明

KiCqStart SYBR Green qPCR ReadyMix是2倍浓缩的即用型预混液，含有除引物和模板之外的实时定量PCR(qPCR)所需的全部组分。这种专有缓冲液、稳定剂和Taq DNA热启动聚合酶的独特组合使用快速或常规的SYBR Green qPCR循环方案，具有最高的PCR效率、灵敏度、特异性和稳定可靠的荧光信号。

高特异性扩增对于使用SYBR Green I染料技术成功进行qPCR至关重要，因为该染料可结合扩增过程中产生的任何dsDNA并进行检测。Taq DNA热启动聚合酶在PCR变性步骤开始之前，处于抗体介导的失活状态。

應用

KiCqStart® SYBRSYB® Green qPCR ReadyMix™ 已用于:

  • 两步 法RT-qPCR 分析从小鼠脑样品来源 RNA 逆转录的 cDNA 的扩增和定量
  • 分析 ChIP 技术纯化的 DNA
  • 基因表达分析
  • 通过定量实时聚合酶链反应 (PCR) 扩增从成年 TL 野生型鱼心脏分离的 RNA
PCR扩增：
  • 基因表达
  • DNA定量
  • 染色质免疫沉淀(CHiP)

特點和優勢

  • 只需33分钟即可获得分析结果
  • 高效、灵敏的实时PCR结果
  • 几乎不需要多少优化，甚至无需优化

成分

2X 反应缓冲液，含有浓度优化的 MgCl2、dNTP、（dATP、dCTP、dGTP、dTTP）、KiCqStart Taq DNA 聚合酶、SYBR Green 染料、ROX 参考染料（用于 580-585 nm 激发）和稳定剂。

包装：
250次反应* = 2 X 1.25 mL试管
1250次反应* = 10 X 1.25 mL试管
5000次反应* = 1 X 50 mL试管
*设一次反应需20uL

其他說明

储存条件：
KiCqStart SYBR Green qPCR ReadyMix在-20°C的恒温冷冻柜中避光保存，可稳定存放1年。为方便使用，也可在+2至+8°C下储存长达6周。解冻后，充分混匀后再使用。不建议反复冻融产品。不过，冻融20次或+20°C存放2个月后，产品没有出现任何性能损失。

法律資訊

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Gene expression study of phase I and II metabolizing enzymes in RPTEC/TERT1 cell line: application in in vitro nephrotoxicity prediction.
Shah H
Xenobiotica, 3, 1-7 (2016)
Gene expression study of phase I and II metabolizing enzymes in RPTEC/TERT1 cell line: application in in vitro nephrotoxicity prediction
<BIG><BIG>Shah H, et al.</BIG></BIG>
Xenobiotica, 47, 837-843 (2017)
Aneesh K Ramaswamy et al.
Matrix biology plus, 4, 100014-100014 (2019-09-04)
Elastogenesis within the medial layer of the aortic wall involves a cascade of events orchestrated primarily by smooth muscle cells, including transcription of elastin and a cadre of elastin chaperone matricellular proteins, deposition and cross-linking of tropoelastin coacervates, and maturation
Eoghan M Cunnane et al.
Bioengineering (Basel, Switzerland), 8(5) (2021-05-01)
Macromolecular components of the vascular extracellular matrix (ECM), particularly elastic fibers and collagen fibers, are critical for the proper physiological function of arteries. When the unique biomechanical combination of these fibers is disrupted, or in the ultimate extreme where fibers
The functional and inflammatory response of brain endothelial cells to toll-like receptor agonists
Johnson RH, et al.
Scientific Reports, 8, 1-12 (2018)
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After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

实验方案

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

KiCqStart™ Universal SYBR® Green qPCR Protocol

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

qPCR Gene Expression/Copy Number Analysis Protocol Using SYBR Green I Dye Detection

Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.

qPCR Reference Gene Selection Protocol

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.

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