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一般說明
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
應用
- insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- mitochondrial gene by conventional PCR
特點和優勢
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
包裝
單位定義
其他說明
法律資訊
相關產品
訊號詞
Warning
危險分類
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
其他客户在看
商品
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
实验方案
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
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