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Merck
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文件

C0549

Sigma-Aldrich

氯仿:异戊醇 24:1

suitable for nucleic acid purification

别名:

chloroform-isoamyl alcohol

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52

形狀

liquid

品質等級

200

包含

0.6-1.0% ethanol as stabilizer

適合性

suitable for nucleic acid purification

應用

agriculture

一般說明

氯仿:异戊醇 24:1混合液是一种用于 DNA 提取和沉淀的有机试剂。

應用

氯仿:异戊醇24:1混合液可用于：

  • 提取细菌基因组 DNA、水蚤(Daphnia) DNA 和鲑鱼精子。
  • 高质量 DNA 提取的单一 DNA 沉淀步骤。
  • 蛋白质提取和 RNA 酶处理。
  • 酚氧化，从水溶液中分离酚类物质。
  • 从棕色脂肪组织 (BAT)、腹股沟白色脂肪组织 (iWAT) 和附睾脂肪组织 (eWAT) 中分离 RNA。
  • 采用改良十六烷基三甲基溴化铵 (CTAB) 方法从凤仙花(Impatiens balsamina)中提取总 RNA 。

訊號詞

Danger

危險分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

185.0 °F

閃點（°C）

85 °C

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异戊醇 natural, ≥98%, FG

Sigma-Aldrich

W205710

异戊醇

氯仿 ≥99%, PCR Reagent, contains amylenes as stabilizer

Sigma-Aldrich

C7559

氯仿

乙酸铵 溶液 for molecular biology, 7.5 M

Sigma-Aldrich

A2706

乙酸铵 溶液

氯仿 contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Sigma-Aldrich

C2432

氯仿

异戊醇 for analysis EMSURE® ACS,Reag. Ph Eur

Supelco

1.00979

异戊醇

氯仿 anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

288306

氯仿

Optimisation of DNA extraction from the crustacean Daphnia.
Athanasio CG
PeerJ, 4, e2004-e2004 (2016)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory
A fragrant neighborhood: volatile mediated bacterial interactions in soil.
Schulz-Bohm K
Frontiers in Microbiology, 6, 1212-1212 (2015)
Ning Jia et al.
Molecular cell, 75(5), 944-956 (2019-07-22)
Type III-A CRISPR-Cas surveillance complexes containing multi-subunit Csm effector, guide, and target RNAs exhibit multiple activities, including formation of cyclic-oligoadenylates (cAn) from ATP and subsequent cAn-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase. Our structure-function studies have focused
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