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Merck

C7559

Sigma-Aldrich

氯仿

≥99%, PCR Reagent, contains amylenes as stabilizer

别名:

三氯甲烷

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About This Item

经验公式(希尔记法):
CHCl3
CAS号:
分子量:
119.38
Beilstein:
1731042
EC號碼:
MDL號碼:
分類程式碼代碼:
12191502
PubChem物質ID:
NACRES:
NA.28
bp:
60.5-61.5 °C (lit.)

等級

PCR Reagent

品質等級

蒸汽密度

4.1 (vs air)

蒸汽壓力

160 mmHg ( 20 °C)

化驗

≥99%

形狀

liquid

包含

amylenes as stabilizer

包裝

vial of 1.5 mL

技術

PCR: suitable

顏色

colorless

折射率

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

密度

1.492 g/mL at 25 °C (lit.)

異物活動

DNase, RNase, none detected

SMILES 字串

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI 密鑰

HEDRZPFGACZZDS-UHFFFAOYSA-N

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一般說明

氯仿在药物制剂中可用作麻醉剂和添加剂。现在它主要用作有机溶剂。氯仿由人为和天然来源产生。这种在大气中含量丰富的卤化碳存在于地下水和地表水源中。

應用

氯仿已用于苯酚-氯仿提取,以考察其从血液培养液中纯化核酸的能力。它还用于 RNA 提取/分离和纯化。

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

does not flash

閃點(°C)

does not flash

個人防護裝備

Eyeshields, Faceshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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