TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Sinonimo/i:
NR uptake assay, Neutral red assay
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.84
Prodotti consigliati
Forma fisica
liquid
impiego
kit sufficient for 1,000 tests
Confezionamento
pkg of 1 kit
Condizioni di stoccaggio
dry at room temperature
λmax
540 nm
applicazioni
cell analysis
detection
Metodo di rivelazione
colorimetric
Temperatura di conservazione
2-8°C
Descrizione generale
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Applicazioni
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Azioni biochim/fisiol
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
73.4 °F
Punto d’infiammabilità (°C)
23 °C
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I clienti hanno visto anche
D Triglia et al.
In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
A new three-dimensional human skin model consisting of several layers of actively dividing and metabolically active human neonatal foreskin-derived fibroblasts and epidermal keratinocytes grown on nylon mesh has been used to assess the in vitro toxicity of test agents from
Guillermo Repetto et al.
Nature protocols, 3(7), 1125-1131 (2008-07-05)
The neutral red uptake assay provides a quantitative estimation of the number of viable cells in a culture. It is one of the most used cytotoxicity tests with many biomedical and environmental applications. It is based on the ability of
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The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology
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One of the most-used food contact materials is stainless steel (AISI 304L or AISI 316L), owing to its high mechanical strength, cleanability, and corrosion resistance. However, due to the presence of minimal crevices, stainless-steel is subject to microbial contamination with
R Fautz et al.
Mutation research, 253(2), 173-179 (1991-10-01)
The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers
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