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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

Sinonimo/i:

mitochondrial activity assay

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.84

impiego

 kit sufficient for 1,000 tests

Livello qualitativo

200

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

λmax

570 nm

applicazioni

cell analysis
detection

Metodo di rivelazione

colorimetric

Temperatura di conservazione

2-8°C

Descrizione generale

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Applicazioni

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Azioni biochim/fisiol

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

Prodotti correlati

N° Catalogo
Descrizione
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Avvertenze

Danger

Indicazioni di pericolo

H225,H314,H335,H336,H341,H410

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

Organi bersaglio

Central nervous system, Respiratory system

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

53.6 °F

Punto d’infiammabilità (°C)

12 °C

Certificati d'analisi (COA)

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Human renal organic anion transporter 1-dependent uptake and toxicity of mercuric-thiol conjugates in Madin-Darby canine kidney cells
Aslamkhan AG, et al.
Molecular Pharmacology, 63(3), 590-596 (2003)
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
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Testicular cell culture of crab, Scylla serrata (Forskal) was used to study the effects of White spot syndrome virus (WSSV). We are showing the susceptibility of cell culture of crabs to WSSV. The proliferating cell culture of testes were maintained
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
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Journal of virology, 91(9) (2017-02-17)
The human immunodeficiency virus type 1 (HIV-1) capsid protein is an attractive therapeutic target, owing to its multifunctionality in virus replication and the high fitness cost of amino acid substitutions in capsids to HIV-1 infectivity. To date, small-molecule inhibitors have
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Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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