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Merck
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Documenti fondamentali

TOX6

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Sulforhodamine B based

Sinonimo/i:

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.84

Stato

liquid

impiego

1 kit sufficient for 1,000 tests

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

λmax

565 nm

applicazioni

cell analysis
detection

Metodo di rivelazione

colorimetric

Temperatura di conservazione

room temp

Descrizione generale

The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.

Applicazioni

In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein. It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines. Absorbance of dye is measured at a wavelength of 565nm.

Azioni biochim/fisiol

Dye binds to cellular protein and is then solubilized in base.

Avvertenze

Danger

Indicazioni di pericolo

H226,H314,H410

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number
SLCK4527
SLCD7118
SLCD0841
SLCB0537
SLBZ2377

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Immune expression and inhibition of heat shock protein 90 in uveal melanoma
Faingold D, et al.
Clinical Cancer Research, 14(3), 847-855 (2008)
Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets
Miranda G, et al.
Neuro-Oncology, 15(2), 172-188 (2012)
P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Jae-Hyeog Choi et al.
Oncoimmunology, 7(5), e1421890-e1421890 (2018-05-04)
Combination therapies with phosphoinositide 3-kinase (PI3K) inhibitors and trastuzumab (anti-human epidermal growth factor receptor [HER]2/neu antibody) are effective against HER2+ breast cancer. Isoform-selective PI3K inhibitors elicit anti-tumor immune responses that are distinct from those induced by inhibitors of class I
Marike Gabrielson et al.
PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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