MAK529

LDH Cytotoxicity Assay Kit

sufficient for 100 colorimetric tests

• Sinonimo/i: LDH-Cytotoxicity Test Kit II
• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• Metodo di rivelazione: colorimetric
• Malattie correlate: hematological disorder; cancer
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme, that upon membrane damage, is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage.

Caratteristiche e vantaggi

• Compatible with high-throughput handling systems.

Compatibilità

The kit is suitable for the rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium, and for the evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.

Principio

The LDH Cytotoxicity Assay Kit is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H302 + H332,H315,H318
• Consigli di prudenza: P261 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3