MAK536
Cytotoxicity Assay Kit
sufficient for 100 assays (bioluminescent)
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
Livello qualitativo
applicazioni
pharmaceutical
Metodo di rivelazione
bioluminescent
Malattie correlate
cancer
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Adenosine 5′-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.
Caratteristiche e vantaggi
- As low as 50 cells can be quantified.
- Compatible with high-throughput handling systems.
- No wash and reagent transfer steps are involved.
Compatibilità
The kit is suitable for:
- Cell proliferation: effects of cytokines, growth factor, nutrients.
- Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
- Drug discovery: high-throughput screening for anticancer drugs.
Principio
The Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability, and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Skin Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
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Certificati d'analisi (COA)
Lot/Batch Number
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