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S8409

Sigma-Aldrich

Superoxide Dismutase from bovine liver

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Sinonimo/i:

SOD, Superoxide: superoxide oxidoreductase

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

ammonium sulfate suspension

Livello qualitativo

Attività specifica

2,000-6,000 units/mg protein (biuret)

PM

32.5 kDa

N° accesso UniProt

Temperatura di conservazione

2-8°C

Informazioni sul gene

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Descrizione generale

Research area: Cell Signaling

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals, and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Applicazioni

Superoxide Dismutase from bovine liver has been used:


  • for measuring mitochondrial hydrogen peroxide production in skeletal muscle
  • as a positive control in Cu(II) binding stability assay
  • as a positive control in superoxide dismutase assay
  • in a study to assess the inactivation of endothelial-derived relaxing factor by oxidized lipoproteins.
  • in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Azioni biochim/fisiol

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Definizione di unità

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Stato fisico

Suspension in 3.8 M (NH4)2SO4, pH 7.0

Risultati analitici

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Applicazioni

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificati d'analisi (COA)

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J H Chin et al.
The Journal of clinical investigation, 89(1), 10-18 (1992-01-01)
Endothelial cell derived relaxing factor (EDRF) mediated relaxation of blood vessels is impaired in vessels exposed to lipoproteins in vitro and in arteries of hyperlipidemic humans and animals. To investigate the mechanism by which lipoproteins impair the effects of EDRF
Molybdenum application enhances antioxidant enzyme activity and COR15a protein expression under cold stress in wheat
Al-Issawi M, et al.
Journal of Plant Interactions, 11(1), 5-10 (2016)
Perturbed redox signaling exacerbates a mitochondrial myopathy
Dogan SA, et al.
Cell Metabolism, 28(5), 764-775 (2018)
E M Link et al.
The Biochemical journal, 249(2), 391-399 (1988-01-15)
1. The survival of mammalian epithelial cells exposed in vitro to the xanthine/xanthine oxidase system in phosphate-buffered saline (PBS) or serum-containing medium (SCMEM) was investigated. 2. The cytotoxic effect observed depended on the composition of the medium in which the
L G Chi et al.
Circulation research, 64(4), 665-675 (1989-04-01)
Available data demonstrate that oxygen free radicals and derived reactive species of oxygen are produced during myocardial ischemia as well as upon reperfusion of the ischemic tissue. The present study was designed to determine if polyethylene glycol-conjugated superoxide dismutase (PEG-SOD)

Articoli

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

Enzymatic Assay of Superoxide Dismutase

Chromatograms

application for HPLC

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